Windows 2008 changed the default encryption level of Kerberos.  They started to disallow DES based Kerberos encryption (because it is not as secure).
In Windows 7 and Windows Server 2008 (might have started in R2, don't remember), you must configure your computers to use the DES-CBC-MD5 or DES-CBC-CRC cipher suites to be compatible with the legacy DES encryption level that the AS400 only supports.
You then have two options:
1. Enable legacy DES encryption for your AS400 by telling your Windows 2008 domain controllers to allow the older, less secure DES encryption method for Kerberos.  See here: 
http://social.technet.microsoft.com/Forums/windowsserver/en-US/80a5845c-aa74-4baf-9c05-8733ffcd0545/windows-2008r2-dcs-enable-use-des-encryption-for-legacy-applications (we enabled it at group policy level so it forced the requirement down to all servers/clients in network under this GPO: Computer Configuration\Policies\Windows Settings\Security Settings\Local Policies\Security Options\Network security: Configure encryption types allowed for Kerberos.
2. (well this isn't an option for you, but for others who are on V5R4 or above can do this): Apply these fixes to allow the AS400 to use more modern Kerberos encryption techniques:
Fix            Release   Description
---------      ---------        ----------------------------
SI42919   V7R1        Adds AES & RC4 encryption support (krb)
SI42957   V6R1        " "
SI43034   V5R4        " "
SI43918   V7R1        Updates KRB5 header file in QSYSINC
SI43919   V6R1        " "
SI43920   V5R4        " "
-----Original Message-----
From: Terry Nonamaker [mailto:TNonamaker@xxxxxxxxxxxxxxxx] 
Sent: Wednesday, June 19, 2013 10:55 AM
To: 'Midrange Systems Technical Discussion'
Subject: RE: Iseries v4r5: QNTC works with Windows 2003 domain but fails with Windows 2008 domain
No, but I am not aware that should make a difference.
-----Original Message-----
From: midrange-l-bounces@xxxxxxxxxxxx
[mailto:midrange-l-bounces@xxxxxxxxxxxx] On Behalf Of Matt Olson
Sent: Wednesday, June 19, 2013 7:16 AM
To: Midrange Systems Technical Discussion
Subject: RE: Iseries v4r5: QNTC works with Windows 2003 domain but fails with Windows 2008 domain
Terry,
Do you use Kerberos for QNTC?
Matt
-----Original Message-----
From: Terry Nonamaker [mailto:TNonamaker@xxxxxxxxxxxxxxxx]
Sent: Tuesday, June 18, 2013 1:26 PM
To: 'Midrange Systems Technical Discussion'
Subject: RE: Iseries v4r5: QNTC works with Windows 2003 domain but fails with Windows 2008 domain
Although this may not sound like your problem ....it was exactly our situation along with extreme slowness ....I would follow this link and give it a try ....this is a post I previously made and I think you will find it useful
Try this.. 
http://archive.midrange.com/midrange-l/201302/msg00431.html
Terry Nonamaker
-----Original Message-----
From: midrange-l-bounces@xxxxxxxxxxxx
[mailto:midrange-l-bounces@xxxxxxxxxxxx] On Behalf Of Stone, Joel
Sent: Monday, June 17, 2013 1:17 PM
To: Midrange Systems Technical Discussion
Subject: Iseries v4r5: QNTC works with Windows 2003 domain but fails with Windows 2008 domain
Running Iseries v4r5
The following command works well with Windows 2003 domain.
We are migrating to Windows 2008 domain (still on v4r5).
CPYTOSTMF  +
             FROMMBR('/qsys.lib/tpgmlib.lib/qclsrc.file/+
             addtimetst.mbr') +
             TOSTMF('QNTC/IGHNAS05/Public_test/GrainMktg+
             /FTP/TEST05') STMFOPT(*ADD) +
             STMFCODPAG(*PCASCII)
Getting security errors when sending to Windows 2008 Domain.
Any ideas how to resolve?
Thanks
      06/17/13  15:06:05.164536  QCADRV       QSYS        03C4     A_TEST05
JSTONE      000C
   Message . . . . :      200 - CPYTOSTMF
     FROMMBR('/qsys.lib/tpgmlib.lib/qclsrc.file/addtimetst.mbr')
     TOSTMF('QNTC/IGHNAS05/Public_test/GrainMktg/FTP/TEST05') STMFOPT(*ADD)
     STMFCODPAG(*PCASCII)
 30   06/17/13  15:06:05.400248  QZLCKERN     QSYS        *STMT    QZLCKERN
QSYS        *STMT
   From module . . . . . . . . :   QZLCVUT
   From procedure  . . . . . . :   QzlcDiagMsg
   Statement . . . . . . . . . :   191
   To module . . . . . . . . . :   QZLCVUT
   To procedure  . . . . . . . :   QzlcDiagMsg
   Statement . . . . . . . . . :   191
   Message . . . . :   Error exchanging security information for user QIJS
on
     Network Server ighnas05.
   Cause . . . . . :   The Network Server file system (QNTC) has encountered
an
               Job Log                             GM400    06/17/13
15:06:07          Page    2
    error when authenticating user QIJS with a Network Server ighnas05.
Recovery
     . . . :   Ensure the following: - The user is set up on Network Server
    ighnas05. - The OS/400 user password matches the Network Server user
    password. - Network Server ighnas05 is enabled for digitally signed
    communications. Technical description . . . . . . . . :   An error has
been
    detected while the Network Server file system was exchanging security
    information with a Network Server. The error class was 2, and the error code
    was 2. Possible error class and error code values include: Class 0 - QNTC
    specifc security error.         1 - QNTC requires digital signing. Class
1 -
    Network Server operating system related error.         1 - Not valid
    Function.         2 - File not found.         3 - Directory not valid.
      4 - Too many files open.         5 - Access denied. Class 2 - Error
    generated by the Network Server.         1 - Non-specific error code.
     2 - Bad password.         3 - Directory not valid.         4 - Access
    denied.         7 - Not valid device. Class 3 - Network Server hardware
    error.        31 - General hardware failure.        39 - No space on
file
From module . . . . . . . . :   QZLCVUT
From procedure  . . . . . . :   QzlcDiagMsg
Statement . . . . . . . . . :   191
To module . . . . . . . . . :   QZLCVUT
To procedure  . . . . . . . :   QzlcDiagMsg
Statement . . . . . . . . . :   191
Message . . . . :   Error exchanging security information for user QIJS on
  Network Server ighnas05.
Cause . . . . . :   The Network Server file system (QNTC) has encountered an
  error when authenticating user QIJS with a Network Server ighnas05.
Recovery
   . . . :   Ensure the following: - The user is set up on Network Server
  ighnas05. - The OS/400 user password matches the Network Server user
  password. - Network Server ighnas05 is enabled for digitally signed
  communications. Technical description . . . . . . . . :   An error has
been
  detected while the Network Server file system was exchanging security
  information with a Network Server. The error class was 2, and the error code
  was 2. Possible error class and error code values include: Class 0 - QNTC
    specifc security error.         1 - QNTC requires digital signing. Class
1 -
    Network Server operating system related error.         1 - Not valid
    Function.         2 - File not found.         3 - Directory not valid.
      4 - Too many files open.         5 - Access denied. Class 2 - Error
    generated by the Network Server.         1 - Non-specific error code.
     2 - Bad password.         3 - Directory not valid.         4 - Access
    denied.         7 - Not valid device. Class 3 - Network Server hardware
    error.        31 - General hardware failure.        39 - No space on
file
    system.
30   06/17/13  15:06:05.621736  QZLCKERN     QSYS        *STMT    QZLCKERN
QSYS        *STMT
  From module . . . . . . . . :   QZLCVUT
  From procedure  . . . . . . :   QzlcDiagMsg
  Statement . . . . . . . . . :   191
  To module . . . . . . . . . :   QZLCVUT
  To procedure  . . . . . . . :   QzlcDiagMsg
  Statement . . . . . . . . . :   191
  Message . . . . :   Error exchanging security information for user QIJS on
   Network Server ighnas05.
 Cause . . . . . :   The Network Server file system (QNTC) has encountered
an
   error when authenticating user QIJS with a Network Server ighnas05.
Recovery
    . . . :   Ensure the following: - The user is set up on Network Server
   ighnas05. - The OS/400 user password matches the Network Server user
   password. - Network Server ighnas05 is enabled for digitally signed
   communications. Technical description . . . . . . . . :   An error has
been
   detected while the Network Server file system was exchanging security
   information with a Network Server. The error class was 2, and the error code
   was 2. Possible error class and error code values include: Class 0 - QNTC
   specifc security error.         1 - QNTC requires digital signing. Class
1 -
   Network Server operating system related error.         1 - Not valid
   Function.         2 - File not found.         3 - Directory not valid.
     4 - Too many files open.         5 - Access denied. Class 2 - Error
   generated by the Network Server.         1 - Non-specific error code.
    2 - Bad password.         3 - Directory not valid.         4 - Access
   denied.         7 - Not valid device. Class 3 - Network Server hardware
error.        31 - General hardware failure.        39 - No space on file
 system.
  06/17/13  15:06:05.721048  QZLCKERN     QSYS        *STMT    QZLCKERN
QSYS
rom module . . . . . . . . :   QZLCVUT
rom procedure  . . . . . . :   QzlcDiagMsg
tatement . . . . . . . . . :   191
o module . . . . . . . . . :   QZLCVUT
o procedure  . . . . . . . :   QzlcDiagMsg
tatement . . . . . . . . . :   191
essage . . . . :   Error exchanging security information for user QIJS on
 Network Server ighnas05.
ause . . . . . :   The Network Server file system (QNTC) has encountered an
 error when authenticating user QIJS with a Network Server ighnas05.
Recovery
  . . . :   Ensure the following: - The user is set up on Network Server
 ighnas05. - The OS/400 user password matches the Network Server user password. - Network Server ighnas05 is enabled for digitally signed
 communications. Technical description . . . . . . . . :   An error has been
 detected while the Network Server file system was exchanging security information with a Network Server. The error class was 2, and the error code was 1. Possible error class and error code values include: Class 0 - QNTC
 specifc security error.         1 - QNTC requires digital signing. Class 1
-
Network Server operating system related error.         1 - Not valid
 Function.         2 - File not found.         3 - Directory not valid.
   4 - Too many files open.         5 - Access denied. Class 2 - Error
 generated by the Network Server.         1 - Non-specific error code.
  2 - Bad password.         3 - Directory not valid.         4 - Access
 denied.         7 - Not valid device. Class 3 - Network Server hardware
 error.        31 - General hardware failure.        39 - No space on file
 system.
From module . . . . . . . . :   QP0LCCMN
From procedure  . . . . . . :
send_STMF_DBF_msg__FPcT1iT1T3PV22qp0l_STMF_DBF
  _commarea
Statement . . . . . . . . . :   16
     /QNTC/IGHNAS05/Public_test/GrainMktg/FTP/TEST05.
   Cause . . . . . :   Object
/QNTC/IGHNAS05/Public_test/GrainMktg/FTP/TEST05, or
     a directory in the object path, could not be found, or its type cannot be
     resolved by this function. Recovery  . . . :   Correct the name or
specify
     an object of the correct type. To determine if the object exists, use the
     Work with Object Links (WRKLNK) command. If the name exists, check the type
     of the object. If the name contains symbolic link objects, ensure the path
     names they resolve to exist.  Retry the operation.
 40   06/17/13  15:06:05.725664  QP0LCCMN     QSYS        *STMT    A_TEST05
JSTONE      000C
   From module . . . . . . . . :   QP0LCCMN
   From procedure  . . . . . . :
send_STMF_DBF_msg__FPcT1iT1T3PV22qp0l_STMF_DBF
     _commarea
   Statement . . . . . . . . . :   109
   Message . . . . :   Object not copied.  Object is
     /qsys.lib/tpgmlib.lib/qclsrc.file/addtimetst.mbr.
   Cause . . . . . :   Object
/qsys.lib/tpgmlib.lib/qclsrc.file/addtimetst.mbr
     was not copied to object QNTC/IGHNAS05/Public_test/GrainMktg/FTP/TEST05
     because errors occurred. Recovery  . . . :   Display the job log
(DSPJOBLOG
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